Nested polymerase chain reaction (nPCR) based diagnosis of bovine leukemia virus in Panama
نویسندگان
چکیده
منابع مشابه
Detection of Mycobacterium avium subspecies paratuberculosis using nested polymerase chain reaction (nPCR)
Mycobacterium avium subspecies paratuberculosis, the cause of paratuberculosis (Johne’s disease) is a chronic debilitating infection in ruminants. The disease is one of the most widespread bacterial infections of ruminants throughout the world. Recently, the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the prese...
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Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...
متن کاملPolymerase Chain Reaction for the Diagnosis of Tuberculosis
Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...
متن کاملUse of the polymerase chain reaction for diagnosing bovine tuberculosis in Panama.
In addition to causing large losses to the cattle industry, Mycobacterium bovis, the causative agent for bovine tuberculosis, is a serious public health issue because it can potentially infect humans. Diagnosis based on isolation and identification of the bacillus is tedious and may take weeks. The diagnosis of M. bovis by polymerase chain reaction (PCR), using species-specific primers, is fast...
متن کاملDirect polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection
Bovine leukemia virus (BLV) infection induces bovine leukemia in cattle and causes significant financial harm to farmers and farm management. There is no effective therapy or vaccine; thus, the diagnosis and elimination of BLV-infected cattle are the most effective method to eradicate the infection. Clinical veterinarians need a simpler and more rapid method of diagnosing infection, because bot...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2017
ISSN: 1684-5315
DOI: 10.5897/ajb2016.15849